原理 および 構造 特性 蛍光 顕微鏡
Fluorescence microscopy uses a point with high luminous efficiency to emit a certain wavelength of light (such as ultraviolet light 3650 in or violet blue light 4200 in) through a color filtering system as excitation light, and excites the fluorescent substances in the sample to emit various colors of fluorescence. After that, it is observed through magnification of the objective lens and eyepiece. In this way, it is easy to recognize even when the fluorescence is very weak against a strong background, with high sensitivity. It is mainly used for the study of cell structure and function, as well as chemical composition. The basic structure of a fluorescence microscope is composed of an ordinary optical microscope plus accessories such as a fluorescent light source, excitation filter, dichroic beam separator, and blocking filter. Fluorescent light sources generally use ultra high pressure mercury lamps (50-200W), which can emit light of various wavelengths. However, each fluorescent substance has an excitation light wavelength that produces the strongest fluorescence, so excitation filters (typically ultraviolet, purple, blue, and green excitation filters) need to be added to allow only a certain wavelength of excitation light to penetrate and irradiate the specimen, while absorbing all other light. After being irradiated by excitation light, each substance emits visible fluorescence longer than the irradiation wavelength in a very short time. Fluorescence has specificity and is generally weaker than excitation light. In order to observe specific fluorescence, it is necessary to add blocking (or suppression) behind the objective lens and use it in combination.
差 間 蛍光 顕微鏡 および 普通 顕微鏡
1. The 照明 方法 is 通常 a 落下 ビーム type, in which the light source is projected onto the sample through an objective lens;
2 です。 光 光源 is 紫外線 光, あり a 短い 波長 および 高い 解像度 通常 顕微鏡;
3 です。そこに ある 2 特殊 フィルター, the one in front of the light source is used to filter out visible light, and the one between the 接眼レンズ and the objective lens is used to filter out ultraviolet rays to protect the human eye。
蛍光 顕微鏡 is also a type of 光学 顕微鏡, with the main difference being the different 励起 波長 this 決定 the differences in structure and usage 間 蛍光 顕微鏡 および 普通 光学 顕微鏡。
